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KMID : 0615720170340020001
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Jouranl of Korean Acupuncture and Moxibustion society
2017 Volume.34 No. 2 p.1 ~ p.18
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Inhibitory Effects of Water Extracts of Eucommiae Cortex and Psoraleae Semen Alone and in Combination on Osteoclast Differentiation and Bone
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Park Jin-Soo
Park Ga-Young
Cho Han-Gyul
Kim Seong-Joung
Kim June-Hyun
Park Min-Cheol
Kim Yun-Kyung
Han Sang-Yong
Jo Eun-Heui
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Abstract
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Objectives: The purpose of this study was to evaluate the effects of water extracts of Eucommiae cortex (EC), Psoraleae semen (PS), and their combination on receptor activator of nuclear factor-kappa-B ligand (RANKL)-induced osteoclast differentiation.
Methods: We assayed the protein expression levels of nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), c-Fos, mitogen-activated protein kinases (MAPKs), and β-actin in cell lysates using western blotting. Similarly, mRNA expression levels of NFATcl, c-Fos, tartrate- resistant acid phosphate (TRAP), and glyceraldehyde-3-phosphate dehydrogenase, spermatogeni (GAPDHS) from bone marrow macrophages (BMMs) were analyzed using reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, we determined the anti-osteoporotic effects of the water extracts of EC, PS, and their combination in a lipopolysaccharide (LPS)-induced bone-loss mouse model.
Results: The in vitro data revealed showed that the combination of EC and PS extract showed a more remarkable inhibition of osteoclast differentiation than each herb did alone. The combination downregulated the induction of c-Fos, NFATc1, and TRAP by suppressing the phosphorylation of p38 and c-Jun N-terminal kinases (JNKs) and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κ B). Lastly, the in vivo data showed that PS reduced the LPS-induced bone erosion.
Conclusion: The result of this study suggests that EC and PS could be potential therapeutic agents for bone loss diseases such as osteoporosis.
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KEYWORD
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Eucommiae cortex, Psoraleae semen, Osteoporosis, Osteoclast differentiation, Bone resorption
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